Abstract
Abstract A quick microtechnique for the determination of the abilities of micro-organisms to produce indole has been described. Heavy inoculations are made into 1 ml. quantities of medium in 10 by 75 mm. tubes that have been preheated to 37 °C. Either tryptone broth or a synthetic medium containing tryptophane has been found to be satisfactory. The tubes are then incubated at 37 °C., preferably in a water bath. Indole may be detected in the tubes upon the addition of 4-drop quantities of a fresh Kovacs solution which has been made with isoamyl alcohol as the solvent. The period of incubation that is necessary has been found to vary from six minutes to two hours, depending upon the strain of organism, the age and size of inoculum, and the method of incubation.
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