Abstract
AbstractA method for estimating free gossypol (FG) has been developed that decreases sample‐determination time from over 2 h to about 25 min per sample. With auto pipetters and bottle‐to dispensers, six sample determinations can be completed in approximately 50 min. The method consisted of adding water and acetone separately to a fixed sample weight, mixing, filtering, diluting with 65% acetone, and reading absorbance on a spectrophotometer. Absorbance was plotted against the official American Oil Chemists’ Society’s FG method for samples that contained FG between 0.02 and 0.9%. Quadratic least squares regression for 31 samples had a correlation coefficient ofr2=0.986 and a standard error of estimated FG of 0.032%.
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