Abstract
In the past submissions of tissue (fine needle biopsies of kidney, liver, incisional biopsies, tumors and miscellaneous other biopsies) for transmission electron microscopy examination were small, i.e. no larger than 1mm cubed (1,2). With such submissions, from the time the specimen was received in the electron microscopy laboratory until photographic prints were available and a diagnosis reported by the pathologist was 72 hrs. A modification of the standard procedure allows the processing of larger and thinner pieces of tissue up to 5mm × 3mm × .5mm and shortens the turn-around time from 72 hrs to 24 hrs.Large pieces of tissue are processed according to the procedure detailed below. All the usual steps are abbreviated: Primary fixation and post-fixation: 5 hrs; dehydration: 20 min; infiltration in epoxy resins: 1 hr and 25 min; embedding and orientation of specimens: 15 min; polymerization: 60 min; cutting thick and thin sections: 50% fewer blocks in comparison to previous procedure; staining in heavy metals: no change.
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