Quick, easy, cheap, effective, rugged and safe strategy for quantifying cadmium polluted rice

Abstract

ABSTRACTCadmium (Cd) pollution is an environmental concern in China. Animals and humans are at risk for Cd poisoning through the consumption of Cd-contaminated rice. A fast and convenient on-site Cd detection tool is required. In this study, we developed a Quick, Easy, Cheap, Effective, Rugged and Safe method to detect Cd in rice. This immunochromatographic method is based on a Cd monoclonal antibody, which recognizes Cd-EDTA complexes. Rice Cd was extracted with 0.1 M HCl under vigorous shaking, transferred to a dispersive solid phase extraction column, and eluted with perchloric acid. Cd recovery was ∼50%. The detection range of our developed method was 0.02–0.8 mg Cd/kg rice with LOD 0.02 mg/kg. The results obtained by immunochromatography which was applied to 71 samples were compared with those obtained via inductively coupled plasma mass spectrometry; the correlation coefficient between these two methods was 0.95. Therefore, our developed method was fast and simple and can be used for on-site Cd detection.