Abstract
Phenols and phenolic compounds are ubiquitous contaminants in the environment. Due to toxic potential, some phenolic compounds mainly chlorophenols and nitrophenols have been classified as priority pollutants. They enter into the environment through various sources such as industrial, domestic and vehicular emissions. For compliance of national and international regulations, various analytical methods have been developed for assessment in the environmental matrices. This paper presents quick, easy and reliable method for simultaneous determination of eleven priority phenolic compounds in wastewater using reversed-phase high-performance liquid chromatography and diode array detector. Liquid-liquid extraction technique with dichloromethane in acidic condition was used for the extraction, and chromatographic separation of compounds was carried out on a C18 column with water and methanol as the mobile phase. The following parameters like selectivity/ specificity, linearity (<em>R</em><sup>2</sup>), range, limit of detection (LOD) and quantification (LOQ), precision (repeatability) and accuracy (recovery) were validated for consistent and reliable results. Calibration curves for all compounds were linear (<em>R</em><sup>2</sup>, 0.998-0.999) within the concentration range of 5-125 (μg/mL). The LOD and LOQ of the method ranged between 0.11- 0.61 μg/L and 0.37-2.04 μg/L, respectively. This method was applied to quantify phenolic compounds in wastewater samples from urban drain with good separated peaks, precision and accuracy.
Highlights
National Reference Trace Organics Laboratory, Central Pollution Control Board, East Arjun Nagar Delhi, India in various groups based on their physicalchemical properties
Acknowledgments: the authors are thankful to the Chairman and Member Secretary, Central Pollution Control Board for the permission and guidance to conduct this work
For compliance of natione al and international regulations, various anas lytical methods have been developed for u assessment in the environmental matrices. l This paper presents quick, easy and reliable ia method for simultaneous determination of eleven priority phenolic compounds in wastec water using reversed-phase high-performance r liquid chromatography and diode array detece tor
Summary
10 μL C18 (4.6×12.5 mm, 5 μm diameter particle size) C18 (4.6×250 mm, 5 μm diameter particle size) (Ascentis®, Supelco, Bellefonte, PA, USA) A (0.1% o-phosphoric acid in HPLC Water) B (0.1% o-phosphoric acid in Methanol) 20 to 95% B in 30 min 0.7 mL/min 25±1°C Diode array detector (DAD), 280 nm mL by vacuum rotary evaporator (Eyela, Tokyo, Japan). Ume of methanol extract was separated on a C18 reversed-phase analytical column (4.6¥250 mm, 5 mm diameter particle) (Ascentis®, Supelco, USA). Gradient flow was set at 0.7 mL/min of 0.1% o-phosphoric acid in methanol and 0.1% o-phosphoric acid in water was used for elution during analysis. Ry with the addition of known quantities of High-performance liquid methanol to 95% in 30 min and come back eleven phenolic compounds in Milli-Q water. Synthetic water sample was adjusted to pH
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