Questions of metrological support for bioanalysis. Catalytic activity of enzymes

Abstract

The relevance of metrological support of measurements carried out in laboratory medicine is examined to increase the accuracy of analyzes results, namely, measurements of the catalytic concentration of the human body enzymes. The development of reference materials with a long shelf life is discussed. The results of the development of Russian reference materials of the catalytic concentration of α-amylase and alkaline phosphatase are discussed. Based on the measuring model (Beer-Lambert-Bouguer law), sources of uncertainty are established. The developed reference materials make it possible to measure the catalytic concentration of enzymes with high reliability, make the correct diagnoses at the early stages of the disease, and make it possible to achieve the world level of metrological support of laboratory medicine in the field of measuring enzyme parameters.