Abstract
In this work of quercetin’s anti-proliferation action on A. flavus, we revealed that quercetin can effectively hamper the proliferation of A. flavus in dose-effect and time-effect relationships. We tested whether quercetin induced apoptosis in A. flavus via various detection methods, such as phosphatidylserine externalization and Hoechst 33342 staining. The results showed that quercetin had no effect on phosphatidylserine externalization and cell nucleus in A. flavus. Simultaneously, quercetin reduced the levels of reactive oxygen species (ROS). For a better understanding of the molecular mechanism of the A. flavus response to quercetin, the RNA-Seq was used to explore the transcriptomic profiles of A. flavus. According to transcriptome sequencing data, quercetin inhibits the proliferation and aflatoxin biosynthesis by regulating the expression of development-related genes and aflatoxin production-related genes. These results will provide some theoretical basis for quercetin as an anti-mildew agent resource.
Highlights
Aspergillus flavus is a saprophytic filamentous fungus that produces aflatoxins (AF), which are mutagenic, teratogenic and carcinogenic toxins for humans and animals [1,2,3,4]
There is a large amount of natural products, synthetic compounds, and extracts from diverse organisms for inhibitors of A. flavus growth, and aflatoxin biosynthesis that were investigated for application in food and feed preservation due to their low impact on the environment and human health [5,6,7,8,9,10]
Quercetin is one of the natural flavonoids that play a crucial role in antibacterial activity [11]
Summary
Aspergillus flavus is a saprophytic filamentous fungus that produces aflatoxins (AF), which are mutagenic, teratogenic and carcinogenic toxins for humans and animals [1,2,3,4]. There is a large amount of natural products, synthetic compounds, and extracts from diverse organisms for inhibitors of A. flavus growth, and aflatoxin biosynthesis that were investigated for application in food and feed preservation due to their low impact on the environment and human health [5,6,7,8,9,10]. The addition of anti-mildew agent is one of the important measures to prevent mildew pollution. Natural anti-mildew agent is a more ideal choice. Quercetin (3,30 ,40 ,5,7-pentahydroxy-flavone) is a natural resource found in many plants, fruits and vegetables [11]. Due to its anti-oxidant [12], anti-inflammatory [13], anti-cancer [5], antiviral, antibacterial [11], and anti-proliferative activity [5,11]
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