Abstract
Purpose: To investigate the effect of Quercetin (Que) on inflammatory reaction in intestinal porcine enterocyte cells, IPEC-J2, induced by lipopolysaccharide (LPS).Methods: IPEC-J2 cells were pretreated with Que and then incubated with LPS. Cell viability, cell morphology, nitric oxide (NO) content, gene expression levels of interleukin-6 (IL-6) and interleukin-8 (IL-8), were then evaluated.Results: LPS incubation at 10 μg/mL for 24 h showed no effect on the viability and cell morphology of IPEC-J2, while Que pre-incubation significantly enhanced the viability of IPEC-J2 and improved cell morphology (p < 0.05). Que decreased the expression levels of pro-inflammatory cytokines IL-6 mRNA and IL-8 mRNA raised by LPS (p < 0.05). Interestingly, both LPS and Que demonstrated no influence on the release of NO.Conclusion: Pre-treatment of Que shows a positive effect on intestinal porcine enterocyte cells and inhibited porcine intestinal inflammation in vitro induced by LPS, but the mechanism may be not associated with NO-related signaling pathway. Therefore, Que might have a potential effect as a veterinary drug or feed additive for the treatment of enteritis in pigs.Keywords: Quercetin, Inflammatory cytokines, Intestinal porcine enterocyte cells, Lipopolysaccharide
Highlights
Severe inflammation of animal intestines has several disadvantages on the livestock and poultry breeding industry, and research on the intestinal inflammation of animals has gradually become the focus of academic field researchers
The cell viability was measured by MTT assay method, and the results showed that 1 to 10 μg/mL of LPS showed no obvious effects on cell viability (p > 0.05) (Figure 1)
Effects of Que on viability and morphology of Intestinal porcine enterocyte cells-jejunum2 (IPEC-J2) incubated with LPS
Summary
Severe inflammation of animal intestines has several disadvantages on the livestock and poultry breeding industry, and research on the intestinal inflammation of animals has gradually become the focus of academic field researchers. The inflammatory response of porcine intestinal tract affects the absorption of nutrients and reduces the growth performance, and decreases the immunity, leading to the infections due to the pathogenic microorganisms, and even death of pigs [1]. Intestinal porcine enterocyte cells-jejunum (IPEC-J2) are normal cells with good biological characteristics of intestinal epithelial cells. These are derived from the jejunal epithelial cells of the piglet and often used as an experimental model in sifting of the functional substances, research of toxicological effects and mechanisms of toxicants, as well as utilization of nutrients [2,3]. In this study, IPEC-J2 were initially pretreated with Que, and treated with LPS, to explore whether or not Que has the potential to inhibit the inflammation of small intestine in pigs
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
