Abstract
A five-herb containing traditional homemade medicine is extensively used to treat gout but has not been standardized for quercetin content. Therefore, the current study describes a reversed-phase liquid chromatographic method for quercetin determination in traditional herbal remedy. The elution was carried out using aqueous 2.0% acetic acid, acetonitrile and tetrahydrofuran (55:40:5, V/V/V) as mobile phase at flow rate of 0.8 mL/min and detection was performed using diode array detector operated at 370 nm. The response of the detector was linear in the range investigated (2.5-160.0 µg/mL) with R2 = 0.996. Results of recovery (98.26-103.22%, SD
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