Quercetin - A Flavonoid Compound from &lt;i&gt;Sarcopyramis bodinieri&lt;/i. var delicate with Potential Apoptotic Activity in HepG2 Liver Cancer Cells

S Zhou,W Huang,C Wan

doi:10.4314/tjpr.v12i4.13

Abstract

Purpose: To isolate and identify the cytotoxicity of the constituents of Sarcopyramis bodinieri var. delicate.Methods: S. bodinieri var. delicate was extracted with hydrochloric acid-methanol and fractionated with ethyl acetate further. The chemical constituents of the ethyl acetate fraction were purified by a combination of D101 macroporous resin and Sephadex LH-20 column chromatography. The structure was characterized by 1H-Nuclear Magnetic Resonance (NMR) and electrospray ionization tandem mass spectrometry (ESI-MS). Apoptosis was evaluated by fluorescence staining and Western blot analysis using 4,6-diamidino-2-phenylindole (DAPI) staining and poly (ADP-ribose) polymerase (PARP) SDSPAGE tests in HepG2 liver cancer cells.Results: One flavonoid with high purity was purified by the combination of D101 macroporous resin and Sephadex LH-20 column chromatography. The flavonoid compound was identified as quercetin by 1HNMR and ESI-MS analyses. DAPI staining and PARP SDS-PAGE tests showed 60 μM quercetin could induce potential apoptotic activity in HepG2 liver cancer cells.Conclusion: Quercetin was the major cytotoxicity constituent in S. bodinieri var. delicate.Keywords: Apoptotic activity, Quercetin, Sarcopyramis bodinieri var. delicate, HepG2 liver cancer cells, Electrospray ionization tandem

Highlights

Sarcopyramis genus belongs to the family of Melastomataceae, containing of 4 species and 2 varietas in china [1]
The ethyl acetate extracts were purified by D101 macroporous resin column chromatography, eluted with a serials of different concentration of ethanol (0%, 30% and 70%, respectively)
Compound 1 was identified as quercetin (1)

Summary

INTRODUCTION

Sarcopyramis genus belongs to the family of Melastomataceae, containing of 4 species and 2 varietas in china [1]. The ethyl acetate extract of of S. bodinieri var. This paper describes a quick method to isolate quercetin, a major active flavonoid compound, from the methanol-HCl extract of S. bodinieri var. Delicate and the evaluation of the apoptotic activity of quercetin in vitro using 4,6diamidino-2-phenylindole (DAPI) staining and poly (ADP-ribose) polymerase (PARP) SDSPAGE tests in HepG2 liver cancer cells. The dried plant material (1 g) was extracted with 20 ml 60 % methanol {with or without 10 % HCl (4:1, v/v)} in a conical flask and refluxed at 80 °C for 2 h. The ethyl acetate extracts were purified by D101 macroporous resin column chromatography, eluted with a serials of different concentration of ethanol (0%, 30% and 70%, respectively). Cells with condensed and fragmented DNA (apoptotic cells) were scored under a fluorescence microscope (Carl Zeiss, Germany) at × 40 objective lens magnification

RESULTS

CONCLUSION

DISCUSSION