Abstract
A novel assay for H2O2 based on luminescence quenching is developed from the Dakin reaction between deprotonated salicylaldehyde (D‐SA) and H2O2. Under alkaline condition, the phenolic hydroxyl group of SA is deprotonated, exhibiting good luminescence emission ability (λex = 433 nm, λem = 508 nm). Luminescence of D‐SA is quenched by H2O2 in a concentration‐dependent manner. Good linearity is obtained with a regression coefficient of 0.9989 in the range of 0.3 ~ 150 μM and a detection limit (LOD) is 30 nM. In addition, D‐SA luminescence probe shows good biocompatibility and low cytotoxicity to HeLa cells through MTT assay. The feasibility for in vitro applications is demonstrated through confocal laser scanning microscopy.
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