QuEChERS sample preparation in the simultaneous determination of residual amounts of quinolones, sulfanilamides, and amphenicols in food using HPLC with a diode-array detector

Abstract

A method is proposed for the simultaneous determination of antibiotics of quinolone series (enoxacin, danofloxacin, lomefloxacin, enrofloxacin, difloxacin, and oxolinic acid), sulfanilamides (sulfanilamide, sulfadiazine, sulfapyridine, sulfamerazine, sulfachloropyridazine, sulfadimethoxine, and sulfaquinoxaline), and amphenicols (chloramphenicol, florfenicol, and thiamphenicol) in foodstuffs by HPLC with diode-array detection at 228 and 280 nm from single weighed portion using a simplified, rapid, and safe QuEChERS sample preparation. The analytical range is 0.01–2 mg/kg for quinolones and 0.002–1 mg/kg for sulfanilamides and amphenicols at a sample weight of 5 g. The relative standard deviation of the results of analysis does not exceed 0.1. The time of analysis is 1–1.5 h.