Abstract
Low fouling positively charged hybrid UF membranes with adjustable charge density fabricated from a blend of PSf/QPSf and GO nanosheets by solution casting and NIPS method. Cross-section SEM image and observed lysozyme transport values at varied pH.
Highlights
The highly efficient puri cation and separation of proteins is important in the eld of bioseparation engineering, for biotechnology, food, biomedical and pharmaceutical industries.[1,2] The complexity of the protein structures, variety of their sequences and folding motives make various approaches inefficient for puri cation and separation of proteins.[3,4] Packed bed column chromatography,[5] affinity membrane chromatography,[6] adsorption,[4] electrophoresis[7] and ultra ltration[3,8] have been explored for puri cation and concentration of proteins from their mixture model solutions and real process samples, with chromatography operations typically being incorporated into downstream processing
Efforts were rendered to increase the loading of graphene oxide (GO) nanosheets from 5 to 7 wt% in the membranes, but pinholes were created a er precipitation in DI water coagulation bath and the membranes became mechanically unstable
Low fouling novel positively charged hybrid ultra ltration membranes were successfully fabricated from blends of PSf and quaternized polysulfone (QPSf) as well as GO nanosheets by a non-solvent induced phase separation method
Summary
The highly efficient puri cation and separation of proteins is important in the eld of bioseparation engineering, for biotechnology, food, biomedical and pharmaceutical industries.[1,2] The complexity of the protein structures, variety of their sequences and folding motives make various approaches inefficient for puri cation and separation of proteins.[3,4] Packed bed column chromatography,[5] affinity membrane chromatography,[6] adsorption,[4] electrophoresis[7] and ultra ltration[3,8] have been explored for puri cation and concentration of proteins from their mixture model solutions and real process samples, with chromatography operations typically being incorporated into downstream processing. Efforts have been made to fabricate hybrid UF membranes with varied charge density from blends of PSf and QPSf polymers with GO nanosheets by solution casting and non-solvent induced phase separation (“phase inversion”) method.
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