Abstract
BackgroundDa Chengqi Tang (DCT) is a common purgative formula in Chinese medicine. Flavanones are its major active compounds derived from Fructus Aurantii Immaturus. The present study developed an LC-MS/MS method to characterize two pairs of flavanone 7-glycoside isomers, i.e., hesperidin versus neohesperidin and naringin versus isonaringin.MethodsAfter solid phase purification, components in sample were separated on a Agilent zorbax SB-C18 (5 μm, 250 mm × 4.6 mm) analytical column. ESI-MS and quasi-MSn were performed in negative ion mode to obtain structural data of these two pairs of flavanone 7-glycoside isomers. Moreover, UV absorption was measured.ResultsThere was no intra-pairs difference in the UV-Vis and MS/MS spectra of the two pairs of 7-glycoside isomers, whereas the mass spectrometry fragmentation pathways between pairs were different.ConclusionThe present study developed a LC-MS/MS method to explore the inter- and intra-pair difference of two pairs of flavanone 7-glycoside isomers.
Highlights
Da Chengqi Tang (DCT) is a common purgative formula in Chinese medicine
Identification of the two pairs of flavanone 7-glycoside isomers by LC-tandem mass spectrometry (MS/MS) We found that hesperidin, neohesperidin and naringin corresponded to the peaks at retention times of 9.3, 10.3 and 8.0 minutes respectively
Mass spectrometric structure characterization of naringin and isonaringin From the first order mass spectrometry scan spectra of naringin and isonaringin (Figure 4A), we found that the [M-H]- ions were both at m/ z 579
Summary
Da Chengqi Tang (DCT) is a common purgative formula in Chinese medicine. Flavanones are its major active compounds derived from Fructus Aurantii Immaturus. Described in Shanghan Lun (Treatise on Cold Damage Diseases, a Chinese medicine classic from the Han dynasty) [1], Da Chengqi Tang (DCT) is a well known purgative formula consisting of Radix et Rhizoma Rhei (Dahuang), Cortex Magnoliae officinalis (Houpu), Fructus Aurantii Immaturus (Zhishi) and Natrii Sulfas (Mangxiao). DCT was found to possess anti-inflammatory effects apart from its purgative activities [6]. Flavanones are a major type of active components in DCT [1,6]. Various studies have revealed a variety of pharmacological activities that citrus flavanones possess, such as (page number not for citation purposes)
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