Abstract
Tremendous interests have been aroused in exploring efficient and readily-prepared barcode particles, with information coding and specific identification features, for biomedical analytical applications. Here, we propose a novel type of barcode for optical encoding and fluorescence enhancement via depositing quantum dots (QDs) on natural pollens intermediated by a polyelectrolyte layer. The exquisite prickly surface morphology and an intrinsic high surface-to-volume ratio of the pollens resulted in a fluorescence enhancement, due to which a much lower detection limit was reached. Besides, the easily-tunable fluorescence peak and intensity of QDs and the repeatable deposition process allowed for the generation of coding combinations for the simultaneous determination of multiple targets. These features endow the QD-coated pollens extraordinary barcodes for applications in multiplexed bioassays.
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