Abstract
The routing of fluorescent signals from NADH to quantum dots (QDs) has been a subject of extensive research for FRET based applications. In the present study, the spectral cross talk of NAD+/NADH with QDs was used to monitor the reaction of NAD+-dependent dehydrogenase enzyme. CdTe QD may undergo dipolar interaction with NADH as a result of broad spectral absorption due to multiple excitonic states resulting from quantum confinement effects. Thus, non-radiative energy transfer can take place from NADH to CdTe QD enhancing QDs fluorescence. Energy routing assay of NADH-QD was applied for detection of formaldehyde as a model analyte in the range 1000–0.01ng/mL by the proposed technique. We observed proportionate quenching of CdTe QD fluorescence by NAD+ and enhancement in the presence of NADH formed by various concentrations of enzyme (0.028–0.4U). Hence, it was possible to detect formaldehyde in the range 1000−0.01ng/mL with a limit of detection (LOD) at 0.01ng/mL and regression coefficient R2=0.9982. Therefore, a unique optical sensor was developed for the detection of the formaldehyde in sensitive level based on the above mechanism. This method can be used to follow the activity of NAD+-dependent enzymes and detection of dehydrogenases in general.
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