Abstract
We have developed a new technique using fluorescent silica nanotubes for simple andsensitive DNA detection. The quantum-dot-embedded silica nanotubes (QD-SNTs) werefabricated by a sol–gel reaction using anodic aluminum silica oxide (AAO) as a template.The fluorescent QD-SNTs of different colors were then immobilized with single-strandedDNA and used as nanoprobes for DNA detection. The optical and structural properties ofQD-SNT nanoprobes were examined using photoluminescence spectroscopy, confocalmicroscopy and transmission electron microscopy (TEM). The QD-SNT nanoprobes wereapplied to detect dye-labeled target DNA in a solution phase. The obvious color change ofthe QD-SNT nanoprobes was observed visually under a simple microscope after thesuccessful detection with target DNA. The quantitative analyses indicated that ∼ 100 attomole of target DNA in one nanoprobe can generate a distinguishable and observablecolor change. The detection results also demonstrated that our assay exhibited highspecificity, high selectivity and very low nonspecific adsorption. Our simple DNA assaybased on QD-SNT nanoprobes is expected to be quite useful for the needs of fast DNAscreening and detection applications.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
