Abstract
To rapidly quantify total immunoglobulin E levels in human serum, we developed a novel quantum-dot-based immunochromatographic assay that employs digital recording of fluorescence. It can detect IgE levels of 5–1000 kU/L, with a coefficient of variation ranging from 2.0 to 9.5%. The assay can be processed in 10 min. The developed assay was tested on 95 serum samples. The correlation coefficient between the IgE values obtained by the proposed assay and those obtained by a commercial ELISA kit was 0.9884. Our assay thus shows promise as a new diagnostic tool for IgE detection.
Highlights
More than a quarter of the world’s population suffers from allergic diseases such as bronchial asthma, allergic rhinitis, and dermatitis
Immunoglobulins of type E (IgE) are located on the surface of mast cells and basophils; upon interactions with allergens they cause cell degranulation, leading to the release of mediators that induce allergic reactions [1,2]
Anti-human IgE antibodies were conjugated with quantum dots by the activated ester technique [35]
Summary
More than a quarter of the world’s population suffers from allergic diseases such as bronchial asthma, allergic rhinitis, and dermatitis. Immunoglobulins of type E (IgE) play a major role in allergen recognition and the initiation of allergic reactions. IgE are located on the surface of mast cells and basophils; upon interactions with allergens they cause cell degranulation, leading to the release of mediators that induce allergic reactions [1,2]. The content of IgE in human blood is less than 0.001% of the total amount of all immunoglobulins [3]. The concentration of total IgE in a healthy adult is about 80 kU/L [6,7]. In the case of allergic disease or myeloma, the IgE content in blood is increased 4–30 fold [8,9]. Methods for determining the total IgE concentration are necessary for primary care providers to assess the state of the immune system and quickly refer patients for further examination
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
