Abstract
We demonstrate that the methods of quantum control can be applied successfully to very large molecules in room temperature liquid solution. Chirped femtosecond pulses are used to excite a green fluorescent protein mutant in both buffered aqueous solution and solid acrylamide gel. At high energy densities, the fluorescence shows a strong chirp dependence, with positively chirped pulses transferring almost 50% more population to the excited state than negatively chirped pulses. By measuring the photobleaching rate in the gel as a function of pulse chirp, we find that the data are consistent with the bleaching of the protein being due to a thermal mechanism rather than to an excited-state photoreaction.
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