Quantitive analysis of flavonoids in ainaxiang tablets by high-performance liquid chromatography

Abstract

Blumea balsamifera is a very famous medicinal plant and functional tea in many countries of Asia. Ainaxiang tablets are a preparation consisting of flavonoids from B. balsamifera. Today, a high performance liquid chromatography method was developed for simultaneous quantification of 15 flavonoids in Ainaxiang Tablets. The analysis was performed on a Kromasil C18 column (250 × 4.6 mm, 5 µm) with a binary gradient mobile phase of acetonitrile and 0.2% aqueous acetic acid. The method was validated in terms of linearity, sensitivity, stability, precision and accuracy. It was found that this method had linearity with R2 at 0.9992- 0.9999 in the test range of 0.15-276.40 µg/mL. The limit of detection (LOD) and limit of quantification (LOQ) for 15 tested reference compounds were 0.01-0.28µg/mL and 0.02 - 0.55µg/mL, respectively. The relative standard deviations (RSD%) for intra-day and inter-day repeatability were not more than 3.18%. The analyzed samples were stable for at least 18 h. The spike recoveries for 15 analyzed samples were 106.17 - 95.02%. The results suggested that this newly developed HPLC method could be used for quantitative analysis of flavonoids in Ainaxiang Tablets and its raw materials.