Abstract

To investigate the potential of dielectric spectroscopy in quantitatively determining the somatic cell count (SCC) of raw milk, the dielectric spectra of 301 raw milk samples at different SCC were collected using coaxial probe technology in the frequency range of 20 to 4,500 MHz. Standard normal variate, Mahalanobis distance, and joint x-y distances sample division were used to pretreat spectra, detect outliers, and divide samples, respectively. Principal component analysis and variable importance in projection (VIP) methods were used to reduce data dimension and select characteristic variables (CVR), respectively. The full spectra, 16 principal components obtained by principal component analysis, and 86 CVR selected by VIP were used as inputs, respectively, to establish different support vector regression models. The results showed that the nonlinear support vector regression models based on the full spectra and selected CVR using VIP had the best prediction performance, with the standard error of prediction and residual predictive deviation of 0.19 log SCC/mL and 2.37, respectively. The study provided a novel method for online or in situ detection of the SCC of raw milk in production, processing, and consumption.

Highlights

  • Mastitis is an inflammatory response caused by udder infection of the dairy cow

  • The SEPP of the built linear support vector regression (LSVR) models with full spectra (FS) (FS-LSVR) and variable importance in projection (VIP) (VIP-LSVR) were 0.22 log somatic cell count (SCC)/mL, lower than 0.24 log SCC/mL of the LSVR model based on principal component analysis (PCA)

  • The dielectric spectra measurement system based on the coaxial probe technology and a milk analyzer were used to collect the dielectric spectra and SCC of 301 raw milk samples, respectively

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Summary

Introduction

Mastitis is an inflammatory response caused by udder infection of the dairy cow. It reduces milk production, degrades milk nutrients, and increases cost due to mastitis treatment (Halasa et al, 2007). There are various methods used in detecting dairy mastitis, including measuring the SCC (Dalen et al, 2019), electrical conductivity (Khatun et al, 2017), and viscosity (Kamphuis et al, 2008) of raw milk, or monitoring body temperature (Hovinen et al, 2008), rumination, and activity (Stangaferro et al, 2016) of cows. To develop rapid and online detection methods, near-infrared (NIR) spectroscopy has been used to measure the SCC of raw milk (Tsenkova et al, 2009; Iweka et al, 2020). The fat globules in raw milk range from 1 to 20 μm in size, causing serious scattering effect on

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