Abstract
"Analytical electron microscope": We have deviced a new analytical electron microscope in Japan for the detection of elemental localization in tissues and cells in 1973.Our analytical EM is basically composed of a JEM 100C transmission electron microscope fitted with a scanning device(ASID-4), a side-entry goniometer stage and an energy dispersive type X-ray microanalyzer unit(EDAX-707B) with an EDIT-Computer system.Freeze substitution method: We compared the results between the freeze dried and the freeze substituted sections of fresh mouse kidney before the cardiac muscle examination. The results were clear that the freeze substitution method with ether or acetone followed by vinylcyclohexane dioxide (ERL-4206) resin mixture seems superior both in the STEM-image and the X-ray microanalysis to that of the freeze dried method.Freshly prepared mouse heart muscle was cut in small pieces within a 3 mm3 in size, mounted on an alminum foil, and frozen with cooled methycyclohexane at the liquid nitrogen temperature, after acrolein vapor fixation for 3 min.
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