Abstract

A method for the complete separation and essentially quantitative isolation of individual components of phospholipid mixtures is described. The mixture is chromatographed in two dimensions on thin layers of Silica Gel G and the phospholipids are extracted from the adsorbent with a solvent mixture containing formic acid.

Highlights

  • MOSTPAPERS appearinq in the literature show thinlayer chromatograms as photoaraphs

  • T h e sensitive side of the blueprint paper is placed down on the thin-layer plate in a manner similar to that used in making a photographic contact print

  • Time of exposure will vary with the density of the plate and the shelf life of the paper

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Summary

Introduction

MOSTPAPERS appearinq in the literature show thinlayer chromatograms as photoaraphs. Eisenberg has described the use of a dry-process blueprint paper for recordin% thin-layer chromatograms (1). T h e thin-layer plate is placed on a n x-ray viewing screen. T h e sensitive side of the blueprint paper is placed down on the thin-layer plate in a manner similar to that used in making a photographic contact print.

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