Abstract
Salinity stress is one of typical abiotic stresses that seriously limit crop production. In this study, a genetic linkage map based on 532 molecular markers covering 1341.1 cM was constructed to identify the loci associated with salt tolerance in Brassica napus. Up to 45 quantitative trait loci (QTLs) for 10 indicators were identified in the F2:3 populations. These QTLs can account for 4.80–51.14% of the phenotypic variation. A major QTL, qSPAD5 on LG5 associated with chlorophyll can be detected in three replicates. Two intron polymorphic (IP) markers in this QTL region were developed successfully to narrow down the QTL location to a region of 390 kb. A salt tolerance related gene Bra003640 was primary identified as the candidate gene in this region. The full length of the candidate gene was 1,063 bp containing three exons and two introns in B. napus L. The open reading frame (ORF) is 867 bp and encodes 287 amino acids. Three amino acid differences (34, 54, and 83) in the conserved domain (B-box) were identified. RT-qPCR analysis showed that the gene expression had significant difference between the two parents. The study laid great foundation for salt tolerance related gene mapping and cloning in B. napus L.
Highlights
Soil salinization is one of the crises the world is facing today
The purpose of this study is to identify the Quantitative trait loci (QTLs) related to salt tolerance, isolate the salt tolerance related genes in the QTL regions, and analyze the structure and expression of the candidate genes
Hydroponic growth was used to cultivate the two parents, F1 and F2:3 populations, in brief, the seeds of them were first disinfected with 70% alcohol, soaked in distilled water for 12 h, and placed in a glass dish to germinate for 48 h under dark conditions
Summary
Soil salinization is one of the crises the world is facing today. Salty soil is a low-yield soil which is widely distributed in the world. It is reported that approximately 37 million hectares of land is exposed to the salinity and secondary salinization in China (Zhang et al, 2007). A large number of rapeseed acreage has been reduced rapidly in China’s Yangtze River Basin because of low profit, which aggravated the gap of China’s edible oil (Yin et al, 2009). Making full use of the saline-alkali soil and planting rapeseed in the saline-alkali soil is one of the most effective ways to alleviate the gap of edible oil in China (Ma et al, 2001). The prerequisite for this approach is to cultivate salt-tolerant rapeseed varieties
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