Quantitative trait locus analysis of four economic traits in one kind of common carp

Abstract

In this study,the reference panel contained 92 individuals,which were a F2 family obtained by mating of F1 hybrids derived from crossing Barbless carp(Cyprinus pellegrini)with Hebao-cold tolerance red carp(Cyprinus carpio wuyuanensis),and the genetic linkage map was constructed with 300 microsatellites,which was used to detect the QTLs for standard length(SL),body thickness(BT),body height(H)and body weight(W)in the panel by GridQTL software.The results showed that 17 related QTLs distributed in the 6 linkage groups were obtained.Four QTLs related to standard length,of which the linkage group of LG5,LG7,LG19 and LG32 were at 5% significant level,but LG32 was at 1% significant level,accounting 5.14%,10.2%,7.02%,9.36% of phenotypic variation,respectively.Four QTLs were identified for body thickness in LG11,LG19,which were at 5% significant level,QTL based on LG5 1% was at significant level,accounting for 6.54%,6.42% and 8.43% of phenotypic variation,respectively.Six QTLs were associated with body height in LG5,LG7,LG10,LG11,LG19 and LG32.LG7 was at 1% significant level,whereas the others were at 5% significant level,accounting 8.95%,6.34%,5.92%,6.29%,6.78%,7.79% of phenotypic variation.Three QTLs were responsible for body weight and LG5,LG7 were at 5% significant level and LG32 was at 1% significant level,which accounted for 6.44%,5.55%,8.36%.Hybrid progenies crossed by two carp strains have been used in this study,which enriched the diversity of QTL population.It is a foundation for further QTL comparative analysis with whole genome level,and common QTL identification in different strains or breeds of carp.It will also be of great assistance in marker-assisted selection(MAS)in common carp.