Abstract
Quantitative analysis of the phospholipid content of pooled CSF and specimens of CSF from some neurological disorders has been investigated by thin layer chromatography. Sensitivity of the Fiske and Subbarow method for the micro determination of phosphorus has been examined and optimum conditions for maximum development of molybdenum blue found to be within closer limits of time and normality than previously reported. Three different methods of extraction of the phospholipids have been investigated. Well-defined chromatograms were obtained from residues of each extraction demonstrating the presence of lysolecithin, lecithin, sphingomyelin and phosphatidylethanolamine, the yields of these substances from each method are compared and a critical analysis of the discrepancies given. Other lysophospholipids and phosphatidylserine were not found. Results obtained in the present work are compared with those from earlier investigations and with the phospholipid content of serum and brain tissue.
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