Quantitative studies of retinal ganglion cells in a turtle, Pseudemys scripta elegans. I. Number and distribution of ganglion cells

Abstract

Multiple pathways for the transmission of visual information from retina to brain have been described in reptiles, but little is known about their functional organization. These parallel channels begin at the retina, and we have therefore begun to study the functional organization of retinal ganglion cells in the turtle, Pseudemys scripta elegans. This paper describes the numbers and distribution of cells in the ganglion cell layer. To develop criteria for the identification of ganglion cells, we labelled them retrogradely by applying horseradish peroxidase (HRP) to the optic nerve. Ganglion cells were found to vary substantially in size and cytology. In low density areas of the retina, ganglion cells typically have cytoplasm with well developed Nissl substance, a distinct, pale nucleus, and a large nucleolus. In high density areas of retina, ganglion cells are small, densely staining, and gliaform. The average minimum proportion of ganglion cells in the ganglion cell layer is 75--80% of total profiles. No more than five or six percent of profiles in the ganglion cell layer are neurons which do not send an axon into the optic nerve (displaced amacrine cells or intraretinal association cells). The ganglion cell layer of P. s. elegans can be divided into a number of regions on the basis of cell density. Isodensity maps constructed from Nissl-stained, wholemounted retinas indicate that there is an elongated region of high ganglion cell density, the visual streak, which extends from nasal to temporal retina and is oriented such that its long axis follows the horizontal axis of the eye. The streak is aligned with the externally visible iris line. Seen in cross-section, the ganglion cell layer in the streak is three to four cells thick; in nonstreak retina, ganglion cells form only a monolayer of somas. Ganglion cell density drops off more rapidly above the streak than below it. The temporal arm of the streak is both shorter and broader than the nasal arm. There is a peak in ganglion cell density at the midpoint of the streak, in the approximate center of the retina. Here, ganglion cell densities exceed 20,000 cells mm-2. The total number of ganglion cells in the retina is 350,000--390,000.