Quantitative Spectrophotometric Estimation of Prednisolone in Tablet Dosage Form Using Eco-friendly Green Solvent and by applying Beer-Lambert’s Law Mathematical Equation Method

Abstract

Prednisolone is a widely used synthetic glucocorticoid drug used in the treatment of allergies, inflammatory conditions, auto-immune disorders, acute and chronic obstructive pulmonary disease, rheumatoid arthritis, systemic lupus erythematosus, dermatitis, eye inflammation, asthma and multiple sclerosis. A simple and economical method was developed using eco-friendly green solvent. In this developed method the eco-friendly green solvent used was ethanol which is available easily. The method was projected to quantitative estimation and assay of Prednisolone in Prednisolone dispersible tablet dosage form. The amount and percent label claim of Prednisolone in tablet dosage form was estimated using Beer-Lambert’s law. Specific absorbance (A1%1cm) of Prednisolone is 415 at lambda max of 243 nm. The method developed was validated as per ICH guidelines. The proposed method was found to be simple, accurate, sensitive, precise, linear and rugged. The method obeys Beer-Lambert’s Law in the concentration range of 2.5-20μg/mL, as the r2 value was found to be 1.000. The mean percent recovery was found to be within acceptable range as per ICH guidelines. Hence the developed method can be used in regular quantitative analysis. The developed method acquires only one green solvent, low cost analytical equipment and the percent label claim can be calculated in the assay of Prednisolone tablets without using any standards solution or standard graph by just employing specific absorbance value in Beer-Lambert’s law, thereby concentration of drug can be calculated. As this method is simple, economical and eco-friendly it can be used elegantly and routinely in analytical laboratories and industries for estimation of Prednisolone in tablet formulation.