Abstract
Polycarbonate filters (Nuclepore Corporation, Pleasanton, CA) have beep used for sizing particulates such as virus particles and liposomes. Unlike nitrocellulose, polyester and other types of filters which are comprised of a relatively thick meshwork of interwoven fibers, polycarbonate filters are thin and contain precisely sized, straight-bore holes (Fig. 1). These features suggest that loss of particles due to nonspecific adsorption or entrapment in the filter matrix might be less for polycarbonate filters. In the present study, we have devised a purification technique based on the sequential filtration of blood plasma through a series of polycarbonate filters. The technique was evaluated by seeding chimpanzee blood plasma with human rotavirus (65-70 nm dia.) and determining recovery of purified virus using a sensitive electron microscope virus particle counting technique.Whole normal blood plasma (10 ml) was seeded with highly purified rotavirus to a final concentration of about 1010 particles per ml.
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Schedule a callMore From: Proceedings, annual meeting, Electron Microscopy Society of America
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