Abstract
A systematic approach using quantitative gas chromatography-mass spectrometry was employed to determine the origin of acetamide and propionamide in microbial pyrograms. Using model compounds, acetamide was shown to be a pyrolysis product of N-acetyl groups on the glycan backbone in cell wall peptidoglycan, and propionamide was shown to be produced from pyrolysis of the lactyl-peptide bridge portion of peptidoglycan. Levels of acetamide and propionamide in the pyrograms of microorganisms and cell wall preparations correlate with the relative amounts of peptidoglycan in these samples, with gram-positive clearly differentiated from gram-negative bacteria.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
