Abstract
BackgroundOsteoporosis (OP) is a systemic bone disease characterized by decreased bone mass, destruction of the bone tissue microstructure, increased bone brittleness and an increased risk of fracture. OP has a high incidence rate and long disease course and is associated with serious complications. Yigu decoction (YGD) is a compound prescription in traditional Chinese medicine that is used to treat OP. However, its mechanism in OP is not clear. This study used a tandem mass tag (TMT)quantitative proteomics method to explore the potential bone-protective mechanism of YGD in an osteoporotic rat model.Materials and methodsA rat model of OP was established by ovariectomy. Eighteen 12-week-old specific-pathogen-free female Wistar rats weighing 220 ± 10 g were selected. The eighteen rats were randomly divided into 3 groups (n = 6 in each group): the normal, model and YGD groups. The right femurs from each group were subjected to quantitative biological analysis. TMT quantitative proteomics was used to analyze the proteins extracted from the bone tissue of rats in the model and YGD groups, and the differentially expressed proteins after intervention with YGD were identified as biologically relevant proteins of interest. Functional annotation correlation analysis was also performed to explore the biological function and mechanism of YGD.ResultCompared with the model group, the YGD group showed significant upregulation of 26 proteins (FC > 1.2, P < 0.05) and significant downregulation of 39 proteins (FC < 0.833, P < 0.05). Four important targets involved in OP and 5 important signaling pathways involved in bone metabolism were identified.ConclusionsYGD can significantly increase the bone mineral density (BMD) of osteoporotic rats and may play a therapeutic role by regulating target proteins involved in multiple signaling pathways. Therefore, these results improve the understanding of the OP mechanism and provide an experimental basis for the clinical application of YGD in OP treatment.
Highlights
Osteoporosis (OP) is a systemic bone disease characterized by decreased bone mass, destruction of the bone tissue microstructure, increased bone brittleness and an increased risk of fracture
The ability of Yigu decoction (YGD) to alleviate OP To explore the ability of YGD to alleviate OP, we first constructed a rat model of OP and measured bone density
The results showed that the bone density value of the model group was significantly lower than that of the normal group (P < 0.01), which indicated that the OP rat model was successfully established
Summary
Osteoporosis (OP) is a systemic bone disease characterized by decreased bone mass, destruction of the bone tissue microstructure, increased bone brittleness and an increased risk of fracture. Yigu decoction (YGD) consists of Fructus psoraleae, Rhizoma Drynariae, Radix Rehmanniae, Salvia miltiorrhiza, Epimedii Folium and Dioscorea opposita. The extract of Salvia miltiorrhiza could improve BMD [17] and increase the levels of alkaline phosphatase and tartrate resistant acid phosphatase (TRACP) [18]. Our team found that YGD could increase the proliferation and differentiation of osteoblasts [22, 23], increase the serum levels of bone Gla protein (BGP) and Ca2+ [24], improve the biomechanical properties of bone [25, 26], and promote the expression of osteoblast BMP [25, 27] to ameliorate the inhibitory effect on bone resorption and achieve the prevention and treatment of OP. Our previous research [26,27,28,29,30] mainly focused on the effect of YGD on the classical Wnt/β-catenin and BMP-2/
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