Quantitative Proteomics Reveals the Dynamic Regulation of the Tomato Proteome in Response to Phytophthora infestans.

Kai-Ting Fan,Yet-Ran Chen,Chi-Hsin Chang,Yang Hsu,Ching-Fang Yeh,Wei-Hung Chang

doi:10.3390/ijms22084174

Abstract

Late blight (LB) disease is a major threat to potato and tomato production. It is caused by the hemibiotrophic pathogen, Phytophthora infestans. P. infestans can destroy all of the major organs in plants of susceptible crops and result in a total loss of productivity. At the early pathogenesis stage, this hemibiotrophic oomycete pathogen causes an asymptomatic biotrophic infection in hosts, which then progresses to a necrotrophic phase at the later infection stage. In this study, to examine how the tomato proteome is regulated by P. infestans at different stages of pathogenesis, a data-independent acquisition (DIA) proteomics approach was used to trace the dynamics of the protein regulation. A comprehensive picture of the regulation of tomato proteins functioning in the immunity, signaling, defense, and metabolism pathways at different stages of P. infestans infection is revealed. Among the regulated proteins, several involved in mediating plant defense responses were found to be differentially regulated at the transcriptional or translational levels across different pathogenesis phases. This study increases understanding of the pathogenesis of P. infestans in tomato and also identifies key transcriptional and translational events possibly targeted by the pathogen during different phases of its life cycle, thus providing novel insights for developing a new strategy towards better control of LB disease in tomato.

Highlights

IntroductionPublisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations
At the stage of biotrophic infection, tomato proteins involved in glycolysis, fatty acid/lipid biosynthesis, and ABA signaling were shown to be upregulated whereas proteins involved in direct defense, redox homeostasis, and jasmonic acid (JA) signaling were repressed
From the biotrophic to transition pathogenesis, proteins involved in JA/ET biosynthesis, JA/salicylic acid (SA) immune signaling, regulation of hypersensitive response (HR), and defense function were upregulated, whereas proteins involved in reactive oxygen species (ROS) production were suppressed

Summary

Introduction

Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations. Late blight (LB), caused by the oomycete Phytophthora infestans, is one of the most notorious plant diseases to afflict solanaceous plants [1]. LB is difficult to control and causes severe losses of up to 100% in the production of potato and tomato crops [2]. LB was the major factor contributing to severe crop loss in Ireland in early 1840, causing the Great

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