Abstract
Disrupted-in-schizophrenia 1 (DISC1) is a key protein involved in behavioral processes and various mental disorders, including schizophrenia and major depression. A transgenic rat overexpressing non-mutant human DISC1, modeling aberrant proteostasis of the DISC1 protein, displays behavioral, biochemical and anatomical deficits consistent with aspects of mental disorders, including changes in the dorsal striatum, an anatomical region critical in the development of behavioral disorders. Herein, dorsal striatum of 10 transgenic DISC1 (tgDISC1) and 10 wild type (WT) littermate control rats was used for synaptosomal preparations and for performing liquid chromatography-tandem mass spectrometry (LC-MS)-based quantitative proteomics, using isobaric labeling (TMT10plex). Functional enrichment analysis was generated from proteins with level changes. The increase in DISC1 expression leads to changes in proteins and synaptic-associated processes including membrane trafficking, ion transport, synaptic organization and neurodevelopment. Canonical pathway analysis assigned proteins with level changes to actin cytoskeleton, Gαq, Rho family GTPase and Rho GDI, axonal guidance, ephrin receptor and dopamine-DARPP32 feedback in cAMP signaling. DISC1-regulated proteins proposed in the current study are also highly associated with neurodevelopmental and mental disorders. Bioinformatics analyses from the current study predicted that the following biological processes may be activated by overexpression of DISC1, i.e., regulation of cell quantities, neuronal and axonal extension and long term potentiation. Our findings demonstrate that the effects of overexpression of non-mutant DISC1 or its misassembly has profound consequences on protein networks essential for behavioral control. These results are also relevant for the interpretation of previous as well as for the design of future studies on DISC1.
Highlights
Disrupted-in-schizophrenia 1 (DISC1) is a gene originally identified as a translocation mutation in an extended Scottish pedigree where carriers suffered from diverse mental disorders comprising schizophrenia and affective disorders (Millar et al, 2000)
Tissue fractionation was initially performed on the dorsal striatum of transgenic DISC1 (tgDISC1) rats to determine the subcellular expression of tgDISC1 and which enrichment steps would be employed in this study (Phillips et al, 2001; Sialana et al, 2016)
Dorsal striata of tgDISC1 rats were fractionated into nuclear/debris, cytosolic, detergent soluble synaptosome (DSS) and postsynaptic density (PSD) preparations
Summary
Disrupted-in-schizophrenia 1 (DISC1) is a gene originally identified as a translocation mutation in an extended Scottish pedigree where carriers suffered from diverse mental disorders comprising schizophrenia and affective disorders (Millar et al, 2000). A second family was later identified with a missense mutation and associated diverse clinical phenotypes (Sachs et al, 2005), and genetic association studies have supported association of DISC1 with mental disorders (Chubb et al, 2008). Insoluble DISC1 protein has been identified in human post mortem brains with mental disorders (Leliveld et al, 2008), indicating that the DISC1 protein can be subject to aberrant proteostasis in vivo. For modeling the effects of aberrant proteostasis in vivo, a transgenic rat model overexpressing (approximately 11-fold) the full length, non-mutant human DISC1 gene (transgenic DISC1, tgDISC1 rat) was generated that exhibited perinuclear aggregates throughout the brain, accentuated in dopamine-rich regions such as in the striatum (Trossbach et al, 2016). At the neuroanatomical level fewer dopaminergic neurons and projections into the dorsal striatum, as well as aberrant interneuron positioning was observed indicating subtle neurodevelopmental disturbance (Hamburg et al, 2016)
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