Abstract
To discover candidate biomarkers for diagnosis and detection of human laryngeal carcinoma and explore possible mechanisms of this cancer carcinogenesis, two-dimensional strong cation-exchange/reversed-phase nano-scale liquid chromatography/mass spectrometry analysis was used to identify differentially expressed proteins between the laryngeal carcinoma tissue and the adjacent normal tissue. As a result, 281 proteins with significant difference in expression were identified, and four differential proteins, Profilin-1 (PFN1), Nucleolin (NCL), Cytosolic non-specific dipeptidase (CNDP2) and Mimecan (OGN) with different subcellular localization were selectively validated. Semiquantitative RT-PCR and Western blotting were performed to detect the expression of the four proteins employing a large collection of human laryngeal carcinoma tissues, and the results validated the differentially expressed proteins identified by the proteomics. Furthermore, we knocked down PFN1 in immortalized human laryngeal squamous cell line Hep-2 cells and then the proliferation and metastasis of these transfected cells were measured. The results showed that PFN1 silencing inhibited the proliferation and affected the migration ability of Hep-2 cells, providing some new insights into the pathogenesis of PFN1 in laryngeal carcinoma. Altogether, our present data first time show that PFN1, NCL, CNDP2 and OGN are novel potential biomarkers for diagnosis and therapeutic targets for laryngeal carcinoma, and PFN1 is involved in the metastasis of laryngeal carcinoma.
Highlights
Laryngeal carcinoma, one of the most common types of cancer in the head and neck, accounts for 2.4% of new malignancies worldwide every year [1,2]
Semiquantitative RT-PCR and Western blotting were performed to detect the expression of the four proteins employing a large collection of human laryngeal carcinoma tissues, and the results validated the differentially expressed proteins identified by the proteomics
The results revealed that the expression of PFN1, NCL, and Cytosolic non-specific dipeptidase 2 (CNDP2) was elevated and that of OGN was reduced in laryngeal carcinoma tissue compared with the adjacent normal tissue (Figure 4B)
Summary
One of the most common types of cancer in the head and neck, accounts for 2.4% of new malignancies worldwide every year [1,2]. This cancer is mainly squamous cell carcinoma, reflecting its origin from the squamous cells [3]. Most patients of laryngeal cancer could retain laryngeal function after the therapy if the disease was detected at an early stage. The fact is that the disease is often diagnosed at advanced stages because of the lack of reliable, early diagnostic biomarkers. Identification of biomarkers for early detection and prognosis is important and may in turn lead to more effective treatments using multiplex technologies
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