Abstract
Most of the microbial cells on earth under natural conditions exist in a dormant condition, commonly known as quiescent state. Quiescent cells exhibit low rates of transcription and translation suggesting that cellular abundance of proteins may be similar in quiescent cells. Therefore, this study aim to compare the proteome of budding yeast cells from two quiescent states viz. stationary phase/G0 and tetrads. Using iTRAQ (isobaric tag for relative and absolute quantitation) based quantitative proteomics we identified 289 proteins, among which around 40 proteins exhibited ±1.5 fold change consistently from the four biological replicates. Proteomics data was validated by western blot and denstiometric analysis of Hsp12 and Spg4. Level of budding yeast 14-3-3 proteins was found to be similar in both the quiescent states, whereas Hsp12 and Spg4 expressed only during stress. FACS (fluorescence-activated cell sorting) analysis showed that budding yeast cells were arrested at G1 stages both in tetrads as well as in stationary phase. We also observed that quiescent states did not express Ime1 (inducer of meiosis). Taken together, our present study demonstrates that the cells in quiescent state may have similar proteome, and accumulation of proteins like Hsp12, Hsp26, and Spg4 may play an important role in retaining viability of the cells during dormancy.
Highlights
Most of the microbial cells on earth under natural conditions exist in a dormant condition, commonly known as quiescent state
Bacterial cells respond to hostile environments like nutrient deficiency and presence of toxic chemicals by forming inert structures commonly referred as bacterial spores, well known for their ability to resist physical and chemical challenge[2,3,4,5]
During entry into the quiescent state, microorganisms undergoes morphological and physiological changes that allows them to resist the effects of environmental stresses
Summary
Most of the microbial cells on earth under natural conditions exist in a dormant condition, commonly known as quiescent state. Cellular abundance of budding yeast Bmh[1] (Brain Modulosignalin Homologue) and Bmh[2] were found to be similar in cells at G0/stationary phase and tetrads, which are important for cell viability under stress and during quiescent stage. Our data suggests that cellular abundance of proteins may be similar in quiescent cells and many of these proteins are important for survival under environmental stress conditions.
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