Abstract
Early planting is one of the strategies used to increase grain yield in temperate regions. However, poor cold tolerance in castor inhibits seed germination, resulting in lower seedling emergence and biomass. Here, the elite castor variety Tongbi 5 was used to identify the differential abundance protein species (DAPS) between cold stress (4 °C) and control conditions (30 °C) imbibed seeds. As a result, 127 DAPS were identified according to isobaric tag for relative and absolute quantification (iTRAQ) strategy. These DAPS were mainly involved in carbohydrate and energy metabolism, translation and posttranslational modification, stress response, lipid transport and metabolism, and signal transduction. Enzyme-linked immunosorbent assays (ELISA) demonstrated that the quantitative proteomics data collected here were reliable. This study provided some invaluable insights into the cold stress responses of early imbibed castor seeds: (1) up-accumulation of all DAPS involved in translation might confer cold tolerance by promoting protein synthesis; (2) stress-related proteins probably protect the cell against damage caused by cold stress; (3) up-accumulation of key DAPS associated with fatty acid biosynthesis might facilitate resistance or adaptation of imbibed castor seeds to cold stress by the increased content of unsaturated fatty acid (UFA). The data has been deposited to the ProteomeXchange with identifier PXD010043.
Highlights
Cold stress is one of the major threats to plant growth, spatial distribution, agricultural productivity, and crop yield [1]
Imbibition is a critical process during seed germination
To identify the differential abundance protein species (DAPS) that contribute to seed germination under cold stress, the changes of proteomic in 12 h early imbibed seeds under cold stress were investigated in this study
Summary
Cold stress is one of the major threats to plant growth, spatial distribution, agricultural productivity, and crop yield [1]. Most temperate plants, such as winter wheat, oats, and barley, can acquire cold acclimation and tolerate ice formation in their tissues; many important crops, such as rice, maize, and soybeans, are sensitive to cold stress and incapable of cold acclimation [2]. Castor bean is sensitive to cold stress in temperate regions, where the temperature drops frequently during the early growing season. A prime target for breeding efforts is to improve seed germination under cold stress. It is imperative to understand the molecular response to cold stress and to identify some novel responsive genes or proteins in castor bean with strong potential for the improvement of cold tolerance by genetic engineering
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
