Abstract
BackgroundMerkel cell carcinoma (MCC) is an aggressive neuroendocrine tumour of the skin with growing incidence. To better understand the biology of this malignant disease, immortalized cell lines are used in research for in vitro experiments. However, a comprehensive quantitative proteome analysis of these cell lines has not been performed so far.MethodsStable isotope labelling by amino acids in cell culture (SILAC) was applied to six MCC cell lines (BroLi, MKL-1, MKL-2, PeTa, WaGa, and MCC13). Following tryptic digest of labelled proteins, peptides were analysed by mass spectrometry. Proteome patterns of MCC cell lines were compared to the proteome profile of an immortalized keratinocyte cell line (HaCaT).ResultsIn total, 142 proteins were upregulated and 43 proteins were downregulated. Altered proteins included mitoferrin-1, histone H2A type 1-H, protein-arginine deiminase type-6, heterogeneous nuclear ribonucleoproteins A2/B1, protein SLX4IP and clathrin light chain B. Furthermore, several proteins of the histone family and their variants were highly abundant in MCC cell lines.ConclusionsThe results of this study present a new protein map of MCC and provide deeper insights in the biology of MCC. Data are available via ProteomeXchange with identifier PXD008181.
Highlights
Merkel cell carcinoma (MCC) is an aggressive neuroendocrine tumour of the skin with growing incidence
Expressed proteins in MCC cell lines and the control cell line The SILAC method was used in six MCC cell lines to determine quantitative changes of proteins at the proteome level
MCC13 was gained from a nodal metastasis of a 80 year old female patient and is called in literature “variant” MCC cell line since unlike BroLi, MKL-1, MKL-2, PeTa and WaGa, it is a Merkel cell polyomavirus negative cell line and lacks some typical markers in immunohistochemical staining [20]
Summary
Merkel cell carcinoma (MCC) is an aggressive neuroendocrine tumour of the skin with growing incidence. Merkel cell carcinoma (MCC) is a rare malignant tumour of the skin with neuroendocrine differentiation [1, 2] and growing incidence rates ranging from 2 to 4 cases per million per year in Europe and the US, to 8 cases per million per year in Australia [3]. Despite of changes at the chromosomal level, the dysfunction of biochemical pathways is expressed at the protein level. This proteomic study was conducted to gain deeper insights into the biology of MCC and possibly
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