Quantitative Profiling of Arabidopsis Polar Glycerolipids under Two Types of Heat Stress.

Feng Qin,Weiqi Li,Yanxia Jia,Liang Lin,Buzhu Yu

doi:10.3390/plants9060693

Abstract

At the cellular level, the remodelling of membrane lipids and production of heat shock proteins are the two main strategies whereby plants survive heat stress. Although many studies related to glycerolipids and HSPs under heat stress have been reported separately, detailed alterations of glycerolipids and the role of HSPs in the alterations of glycerolipids still need to be revealed. In this study, we profiled the glycerolipids of wild-type Arabidopsis and its HSP101-deficient mutant hot-1 under two types of heat stress. Our results demonstrated that the alterations of glycerolipids were very similar in wild-type Arabidopsis and hot-1 during heat stress. Although heat acclimation led to a slight decrease of glycerolipids, the decrease of glycerolipids in plants without heat acclimation is more severe under heat shock. The contents of 36:x monogalactosyl diacylglycerol (MGDG) were slightly increased, whereas that of 34:6 MGDG and 34:4 phosphatidylglycerol (PG) were severely decreased during moderate heat stress. Our findings suggested that heat acclimation could reduce the degradation of glycerolipids under heat shock. Synthesis of glycerolipids through the prokaryotic pathway was severely suppressed, whereas that through the eukaryotic pathway was slightly enhanced during moderate heat stress. In addition, HSP101 has a minor effect on the alterations of glycerolipids under heat stress.

Highlights

Heat stress, which is one of the most common abiotic stresses experienced by plants, can affect the distribution of wild plants and the production of crops [1,2,3,4,5]
Heat acclimation led to a decrease in some and hot-1 plants under two types of heat stress, heat shock (HS; 45 °C, 3 h), and moderate heat stress classes of glycerolipids, it reduced the degradation of glycerolipids under heat shock
Our results demonstrated that heat acclimation (38 ◦ C, 2 h) led to decreased contents of monogalactosyl diacylglycerol (MGDG) and PG, MGDG, in both wild-type Arabidopsis and hot-1

Summary

Introduction

Heat stress, which is one of the most common abiotic stresses experienced by plants, can affect the distribution of wild plants and the production of crops [1,2,3,4,5]. According to the extent and rate of the change in temperature, heat stress can be classified into heat shock and moderate heat stress [6,7]. A temperature increase of 3–4 ◦ C could cause crop yields to fall by. 15%–35% in Africa and Asia and by 25%–35% in the middle east [9]. Field experiments have indicated that an increased temperature will have a net negative impact on the yield of rice in tropical/subtropical. An increase in temperature of only 1 ◦ C in the wheat-growing season reduces wheat yields by 3%–10% in China [11]. Due to the escalating adverse impact of high temperature on agricultural crop production, we should investigate all aspects of the underlying mechanisms related to heat stress

Methods

Results

Discussion

Conclusion