Abstract
Abstract The benzyl esters of short and medium chain fatty acids (C1—C12) were prepared with N,N′-dicyclohexyl-O-benzylisourea or with phenyldiazomethane. The extent of estertification was checked by using 14C-labeled fatty acids. The fatty acid benzyl esters were separated gas chromatographically on both SE-30 and EGSS-X columns. For quantification, the relative response for equal weights of fatty acids was determined. The mild esterification with phenyldiazomethane allows this method to be used for the assay of short and medium chain free fatty acids in biological materials.
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