Abstract
Summary The composition and immunologic reactions of sulfated mucopolysaccharide antigens from chicken chorioallantoic fluid were examined. Differences in NANA content between host antigen (HF) and saccharides from both vaccine supernatant fluid and from normal CA fluid were ascribed to the preparative methods used. Reduction of disulfide bonds altered the immunologic reactivity of HF without changing its qualitative analytical properties. The possible contributions of NANA, fucose and protein to the antigenic determinants of these macromolecules are considered.
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