Quantitative Phosphoproteomic Analysis Provides Insight into the Response to Short-Term Drought Stress in Ammopiptanthus mongolicus Roots.

Huigai Sun,Bolin Xia,Yijun Zhou,Fei Gao,Xue Wang

doi:10.3390/ijms18102158

Huigai Sun, Bolin Xia + Show 3 more

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https://doi.org/10.3390/ijms18102158

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Abstract

Drought is one of the major abiotic stresses that negatively affects plant growth and development. Ammopiptanthus mongolicus is an ecologically important shrub in the mid-Asia desert region and used as a model for abiotic tolerance research in trees. Protein phosphorylation participates in the regulation of various biological processes, however, phosphorylation events associated with drought stress signaling and response in plants is still limited. Here, we conducted a quantitative phosphoproteomic analysis of the response of A. mongolicus roots to short-term drought stress. Data are available via the iProx database with project ID IPX0000971000. In total, 7841 phosphorylation sites were found from the 2019 identified phosphopeptides, corresponding to 1060 phosphoproteins. Drought stress results in significant changes in the abundance of 103 phosphopeptides, corresponding to 90 differentially-phosphorylated phosphoproteins (DPPs). Motif-x analysis identified two motifs, including [pSP] and [RXXpS], from these DPPs. Functional enrichment and protein-protein interaction analysis showed that the DPPs were mainly involved in signal transduction and transcriptional regulation, osmotic adjustment, stress response and defense, RNA splicing and transport, protein synthesis, folding and degradation, and epigenetic regulation. These drought-corresponsive phosphoproteins, and the related signaling and metabolic pathways probably play important roles in drought stress signaling and response in A. mongolicus roots. Our results provide new information for understanding the molecular mechanism of the abiotic stress response in plants at the posttranslational level.

Highlights

IntroductionPlants frequently suffer from adverse environmental factors in their lifetimes
As sessile organisms, plants frequently suffer from adverse environmental factors in their lifetimes
The motif analysis revealed that many phosphorylation sites in differentially-phosphorylated phosphoproteins (DPPs) were binding sites of SnRK2, the key kinases of abscisic acid (ABA) signaling pathways, highlighting the role of the ABA signaling in drought stress response in A. mongolicus roots

Summary

Introduction

Plants frequently suffer from adverse environmental factors in their lifetimes. Of the various environmental stresses, drought is one of the factors that negatively affects plant growth and development, as well as crop yields and quality. Extensive studies using transcriptomic and proteomic approaches have been performed to investigate the plant responses to drought stress, and these works led to the identification of a large number of stress-responsive genes/proteins [2,3]. Further characterization of these stress-responsive genes/proteins promotes our understanding of the abiotic stress regulatory networks in plants. Stress signaling in plants results in extensive biochemical and physiological alteration, as well as gene regulation

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