Abstract
We demonstrate a new method of measuring quantitative phase in imaging of biological materials. This method, asynchronous digital holography, employs knowledge of a moving fringe created by acousto-optic modulators to execute phase-shifting interferometry using two near-simultaneous interferograms. The method can be used to obtain quantitative phase images of dynamic biological samples on millisecond time scales. We present results on a standard sample, and on live cell samples.
Highlights
Quantitative phase measurements have merited great interest lately for measuring cellular dynamics [1,2]
The system uses asynchronous digital holography (ADH), a phase referencing method based on acousto-optically generated moving fringes, to perform quantitative phase microscopy
We show that ADH obtains quantitative phase images on the 10 ms time scale, and demonstrate its application to several biological systems
Summary
Quantitative phase measurements have merited great interest lately for measuring cellular dynamics [1,2]. One natural modality for studying these dynamics is digital holographic interferometry (DHI) [3], a simple interferometric configuration that can be used to assess subwavelength changes in an object by measuring the optical path length difference between each imaged point at two distinct times. There are complications, in utilizing DHI at a high enough speed to visualize cell dynamics, usually considered to be ~10 ms. The system uses asynchronous digital holography (ADH), a phase referencing method based on acousto-optically generated moving fringes, to perform quantitative phase microscopy. We show that ADH obtains quantitative phase images on the 10 ms time scale, and demonstrate its application to several biological systems
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
