Abstract
Red blood cells (RBCs) have the ability to undergo morphological deformations during microcirculation, such as changes in surface area, volume and sphericity. Optical waveguide trapping is suitable for trapping, propelling and deforming large cell populations along the length of the waveguide. Bright field microscopy employed with waveguide trapping does not provide quantitative information about structural changes. Here, we have combined quantitative phase microscopy and waveguide trapping techniques to study changes in RBC morphology during planar trapping and transportation. By using interference microscopy, time-lapsed interferometric images of trapped RBCs were recorded in real-time and subsequently utilized to reconstruct optical phase maps. Quantification of the phase differences before and after trapping enabled study of the mechanical effects during planar trapping. During planar trapping, a decrease in the maximum phase values, an increase in the surface area and a decrease in the volume and sphericity of RBCs were observed. QPM was used to analyze the phase values for two specific regions within RBCs: the annular rim and the central donut. The phase value of the annular rim decreases whereas it increases for the central donut during planar trapping. These changes correspond to a redistribution of cytosol inside the RBC during planar trapping and transportation.
Highlights
Red blood cells (RBCs) scaffolding enables RBCs to behave like a fluid and squeeze themselves through capillaries.[1]
We developed a methodology to trap and propel RBCs using planar optical waveguides and used quantitative phase microscopy to simultaneously detect the response of RBCs during planar trapping and propulsion
Several challenges associated with successful implementation of waveguide trapping (WT) with quantitative phase microscopy (QPM) have been
Summary
RBC scaffolding (cytoskeleton) enables RBCs to behave like a fluid and squeeze themselves through capillaries.[1] A decrease or loss of RBC deformability is associated with multiple diseases such as malaria, sickle cell anaemia, diabetes, cardiovascular disease, and hypertension. A loss of RBC deformability has been reported during blood storage.[8]. Deformation of the red blood cells has been used for studying several diseases previously. Previous studies on RBC deformability have utilized techniques such as microfluidics[17] or optical tweezers[15,18] to simulate some of the forces encountered in vivo. A tightly focused laser beam uses force from the refractive bending of light to trap biological
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