Abstract
It is now just 10 years since the first published description of a method for elemental analysis of biological fluids by electron probe (1). During this decade microdroplet analysis techniques have been highly perfected and are in routine use in many laboratories for the study of droplets in the 10 to 100 picoliter (pi) range. Despite this rapid development, droplet analysis remains almost entirely elemental since techniques for molecular characterization by electron probe have not been established. In renal physiology, where microdroplet analysis has been most accepted, single specimens are routinely studied for Na, K, Ca, Mg, Cl, P, and S; but urea, which plays a fundamental role in the mechanism of urine concentration, cannot be directly measured except by wet photometric techniques requiring several nanoliters of fluid and many quantitative handling steps (2, 3). In this report, a new method for droplet analysis is presented which permits electron probe measurement of both urea and elemental concentrations in single 50 pi droplets.
Published Version
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