Abstract
Quantitative NMR spectroscopy can be applied to the quality assessment of drugs, agrochemicals, and foods, for example, in impurity profiling and determination of the content; the characterization of vaccines and polymers, for example, excipients in drug formulations; as well as the metabolic profiling of drugs in body fluids, using 1H, 13C, 19F, 15N, or 31P NMR spectroscopy. Since the intensity of a signal is directly proportional to the number of nuclei N evoking the signal, the quantification can be performed by simply measuring the area under a signal. This article will give the rules and parameters that have to be considered for precise measurements.
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