Abstract
Quantitative interpretation of the localizations found in single molecule localization based super-resolution is hindered by lack of knowledge of the labeling efficiency. For example, to quantify the number of constituents in clusters of membrane proteins, one must know what percentage of these constituents are fluorescently labeled. In this work, we demonstrate that the efficiency of primary antibody labeling of membrane proteins can be estimated with a two-target super-resolution experiment combined with an analysis that can find the percentage of doubly-labeled proteins.
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