Quantitative microscopical and histochemical study of the skin of mice under the effect of exposure to ultra violet rays type-B

Abstract

The modern industrial era, has benefits and also disadvantages. The main disadvantages are environmental pollution and ozone layer depletion. Ozone layer depletion leads to failure of absorption and prevention of harmful rays from penetration of earth atmosphere. The current work studied the effect of ultra violet-B rays on the skin of mice. Twenty-four mice were subjected to ultra violet-B rays in dose of 1.4J/cm2 for 15minutes every other day for 10weeks. Paraffin sections slides were prepared and stained with suitable histological stains to study the morphology, collagen and elastic fibers, mast cells and glycosaminoglycans materials in the skin. The study included quantitative morphometric analysis by image analyzer on some obtained data. The study revealed a statistically significant increase in the epidermal thickness (p<0.001), associated with necrotic cells, compact ortho-keratosis in the stratum corneum. A degenerated collagen fibers was observed in exposed dermal skin with statistically significant decrease in optical density of collagen fibers (p<0.001). There was a loss of elastic fibers fibrillary pattern. Also there was an increase in melanin pigments concentrations, mast cells, deposition of PAS positive materials in the basement membrane at dermo-epidermal junction, around blood vessels, sebaceous and sweat glands and hair follicles. The study concluded that there was a defined pathological change in the skin exposed to ultraviolet rays and adequate protective prophylactic application must be used to minimize the effect of ultraviolet radiations exposures.