Quantitative microbiological slaughter process analysis in a large-scale Swiss poultry abattoir

Abstract

We performed a quantitative microbiological slaughter process analysis in a large-scale Swiss poultry abattoir. At each of six selected process steps (after plucking, cloaca excision, evisceration, washing, water spray chiller and dry air chiller), 103 carcasses from 50 flocks were sampled and quantitatively tested for indicator bacteria (total viable counts, Escherichia coli/Enterobacteriaceae counts) as well as for Campylobacter spp. and extended spectrum betalactamase (ESBL)-producing Enterobacteriaceae. Samples consisted of a pooled back, breast and leg skin sample as well as swab samples from the thoracic and abdominal cavities. On skin samples, the presence of Escherichia coli and Enterobacteriaceae as indicators of fecal contamination increased between plucking and evisceration (on average by 1.2 log CFU/g), while washing after evisceration successfully decreased the microbial load (on average by 0.8 log CFU/g). Subsequent chilling slightly reduced microbial counts on the carcasses skin and markedly reduced the counts in the abdominal cavity. ESBL-producing Escherichia coli was present above the limit of detection (LOD) with a prevalence of 6.1 %, the majority of which belonged to phylogenetic group A. The blaCTX-M-1 gene was found in all ESBL-producing strains. The prevalence of Campylobacter above the LOD was 16.7 %, and none of the final, chilled carcasses had counts that exceeded 1000 CFU/g. Multilocus sequence typing of 120 Campylobacter jejuni strains yielded 15 different sequence types (ST) among which ST 21 was predominant with 31.7 % and four newly defined ST.