Abstract
The bromoacetyl cellulose immunoadsorbant has been adapted for use as a quantitative micro-method for the measurement of antibody in small amounts of serum (0·1–0·2 ml). The technique is sensitive enough to measure 25 μg antibody/ml serum with an error of ±5 per cent. Smaller amounts of antibody can be measured under closely controlled conditions, but with a greater degree of error. The use of this method circumvents many of the difficulties involved in measuring small amounts of antibody by the precipitin reaction or by indirect, relative methods. The immunoadsorbant micro-technique is especially useful with synthetic polypeptide antigen systems.
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