Abstract
A study was conducted in rats to develop procedures suitable for quantitative measurement of inhibition of aliesterases by organic phosphate insecticides. A suitable manometric procedure using diethylsuccinate and tributyrin as substrates was developed for this purpose. Acylamidase was measured by a previously developed method with acetanilid as the substrate. The applicability of these procedures for measuring aliesterase inhibition was demonstrated by feeding dietary levels of 0, 0.2, 1, 5, and 25 ppm of EPN and Delnav for 13 weeks. Aliesterase enzyme assays were performed on liver and serum by sacrificing animals at intervals during the feeding period, and cholinesterase activity was measured on these tissues and on brain. Maximal inhibition of aliesterase activity occurred early in the feeding period, and a dose-related response was obtained with both compounds. Aliesterases were much more sensitive to inhibition by EPN and Delnav than cholinesterase.
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